FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003249725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698567
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC135380.2881304023120241No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA104290.22196129160231196No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC77440.1648162088568706No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC74690.15896336053098742No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC74590.15875052968277348No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC69490.14789615642386283No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA69020.14689585143725736No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC68700.14621479272297278No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA67200.14302232999976378No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC65840.14012783046405425No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT62960.13399830203549296No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC61790.13150818111138993No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT60730.12925217412032222No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC59720.1271025825533615No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA59710.1270812994685401No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT59330.12627254224532716No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA59100.1257830312944351No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA57110.1215476974149778No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC56390.12001531530783746No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG55410.11792957299534093No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC53190.11320472816499158No Hit
CCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTC52440.11160849680338708No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT52370.11145951520963732No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC49860.10611746091946758No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG48460.1031378290444725No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC48190.10256318575429486No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT47550.1012010683257257No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC47130.10030717876322719No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)