FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003249738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810644
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG75260.26776781406681177No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA72330.25734315694196774No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT71820.25552862617962285No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG69610.24766565954279515No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT68950.24531744326211358No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC64250.22859529702089626No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC48500.17255831759554038No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG46890.16683009303205953No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC38020.13527148938108136No Hit
GCTAGTTGTAGGAGCAGAGGTTGTGCTCGTGGTGGGAACAGGGCTGAGAG37780.1344175925517426No Hit
GCTGGTTGTAAGGGGAGAGATTGTGCTGGTTGTAGAAGCAGAGGTTGTGC35590.12662578398402644No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG35300.12559399198190876No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA35060.12474009515257001No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA34430.12249861597555577No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT33490.1191541867273123No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA32680.11627228492829401No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG32080.11413754285494714No Hit
CAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCA31440.1118604846433771No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT31080.11057963939936898No Hit
GCTGGTTGTAGGGGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGC29010.10321477924632219No Hit
GGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAA28630.1018627759332025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)