FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003249747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746646
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA147330.5363996670848737No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT135460.49318332249587316No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC85550.31147079019283885No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT80070.29151918376084873No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT73360.2670893882939411No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC70770.2576597056919603No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC65150.2371983866868901No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC65110.23705275452315294No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA63370.23071775540058673No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT60140.21895795817881156No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC58170.2117855741147567No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT56860.2070161207523649No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA56290.20494086241911041No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA55810.2031932764542646No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT55280.20126365028474727No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG54620.19886071958308424No Hit
GCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCG52100.1896858932676435No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT52080.18961307718577494No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA50940.18546256051926605No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC49730.1810571875662171No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG49400.1798557222153856No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC48820.17774405584119687No Hit
CAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGA47660.17352072309281938No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC46260.168423597362019No Hit
GCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT45350.16511046563699872No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC44460.16187014999384702No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT43960.16004974794713261No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC43680.15903032280097254No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC43390.15797448961387817No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC43280.15757400116360099No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC43200.15728273683612667No Hit
ACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCT42750.15564437499408368No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG42680.15538951870754367No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG42180.15356911666082926No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT41890.15251328347373486No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT41040.14941859999432033No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA39760.1447583707547314No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA39490.1437753536495056No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG39270.14297437674895128No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC38810.14129960686597398No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA38720.1409719344975654No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT38690.14086271037476253No Hit
CTTGGCTGTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAG38140.13886026812337665No Hit
CTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACA37790.13758598669067656No Hit
TCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGA37340.13594762484863357No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT37140.1352194640299478No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC37060.1349281997024735No Hit
CGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATG36620.13332624590136477No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC36580.13318061373762763No Hit
CCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCAC35510.12928495335765877No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA35490.12921213727579017No Hit
GCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTG35380.12881164882551302No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC35230.12826552821149867No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC35050.12761018347468148No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA34720.12640871812384996No Hit
CACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCA33340.12138440847491813No Hit
CCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCT33220.12094751198370667No Hit
CTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAA33120.12058343157436378No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC32940.1199280868375466No Hit
GGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCAT32690.11901788581418937No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC32570.1185809893229779No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA32450.11814409283176644No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG32360.11781642046335786No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC32280.11752515613588355No Hit
AAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGA32250.11741593201308068No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG32150.1170518516037378No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG31850.11595961037570914No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT31840.11592320233477485No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT30800.11213676607760885No Hit
AGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCAC30710.11180909370920025No Hit
GATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTC30560.11126297309518592No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT30470.11093530072677732No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC30210.1099886916624858No Hit
GATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACC30040.10936975496660291No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCT30030.10933334692566862No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT29920.10893285847539144No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTG29860.1087144102297857No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG29580.10769498508362563No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT29460.10725808859241416No Hit
CACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGA29120.10602021520064835No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAG29100.10594739911877979No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC29090.10591099107784549No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC29080.1058745830369112No Hit
AAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAG28950.10540127850476545No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT28860.10507360613635686No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC28080.10223377894348235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)