FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308863631
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4487200.010.7618991
TCGGAAG4544750.010.6293332
CGGAAGA4652700.010.4339253
AGAGCAC5857300.08.5417727
AGCACAC6113250.08.1877019
GAGCACA6242150.08.095268
GAAGAGC6471050.07.80778655
TATGCCG2950050.07.19365023
ATGCCGT3033600.07.04808764
GCCGTCT3148950.06.863566
TGCCGTC3162600.06.82247835
CGTCTTC3384900.06.4664918
AAGAGCA8019250.06.42965566
GGAAGAG8495950.06.17382344
CCGTCTT3674350.06.0064017
GTATGCC3880700.05.6105532
CGTATGC3183650.05.0767431
Produced by FastQC (version 0.11.5)