FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331005408
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG7965820.24065528258680297TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA7719700.016.5727123
TCGGAAG7789350.016.4375572
ATCGGAA7830100.016.2414681
AGAGCAC8458150.015.2604977
AGCACAC8521900.015.2016169
GAGCACA8741000.014.8156018
GAAGAGC9393550.013.8411895
AAGAGCA10896700.012.0495666
GGAAGAG11960500.011.0587964
TATGCCG2714150.09.59190445-49
CTCGTAT2706800.09.36263440-44
ATGCCGT2791250.09.32747545-49
TCTCGTA2777000.09.12699840-44
TGCCGTC2917800.08.88018245-49
GCCGTCT2915900.08.83651650-54
TATCTCG2907700.08.64093740-44
CGTATGC3003100.08.63277845-49
TCGTATC3054700.08.47507235-39
CGTCTTC3137850.08.44024650-54
ATCTCGT3044600.08.35861440-44
Produced by FastQC (version 0.11.5)