FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300964552
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG8945510.2972280270402077TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC11982250.012.0924949
AGAGCAC12185750.011.8703297
GAGCACA12251450.011.8149558
CGGAAGA12298750.011.685783
TCGGAAG12487450.011.5251772
ATCGGAA12733800.011.2431251
GAAGAGC13294250.010.9422925
AAGAGCA14447300.010.1456156
GGAAGAG15701600.09.3906424
TATGCCG3887650.07.57043345-49
ATGCCGT3924700.07.47199545-49
GCCGTCT3940200.07.34880650-54
TGCCGTC4009750.07.289277645-49
CGTCTTC4077100.07.25177950-54
CTCGTAT3915700.07.241317340-44
TCTCGTA4017600.07.084361640-44
CGTATGC4205150.06.90953245-49
CCGTCTT4413750.06.731845450-54
ATCTCGT4285700.06.65201340-44
TCGTATG4324250.06.627922540-44
Produced by FastQC (version 0.11.5)