FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477506682
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG999150.040.551269
ATCGGAA1463250.028.5889191
GAGCGTC1435050.028.5556248
TCGGAAG1646900.025.4657232
AGAGCGT1737850.023.748437
CGGAAGA1853700.022.6005233
AAGAGCG2113350.019.748356
CGCCGTA663750.012.31283455-59
TCGCCGT800750.010.33840855-59
GCCGTAT925750.09.20399855-59
GAAGAGC5294450.08.4921395
CCGTATC1045300.08.19988855-59
GTCGCCG1033150.08.15458855-59
CGTATCA1130700.07.62430160-64
CGTCGTG1104600.07.622293510-14
TGGTCGC1162100.07.45653750-54
GCGTCGT1163550.07.26976310-14
GTGGTCG1265150.06.942000450-54
GGTCGCC1285050.06.779217750-54
TCCGATC372650.06.7115563
Produced by FastQC (version 0.11.5)