FastQCFastQC Report
Thu 8 Dec 2022
EGAF00003249796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460648563
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG331502037.1964194969170014TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCCCGTATG5782430.12552801559482993TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTATG4830170.10485585732740038TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)