FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393223932
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT4075180.1036350961466913Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1397900.048.837659
GAGCGTC1707500.040.1391038
ATCGGAA1995150.035.518851
AGAGCGT2019800.034.1462027
TCGGAAG2109850.033.31592
CGGAAGA2312650.030.3573383
AAGAGCG2410750.029.0807826
CGCCGTA851150.016.43279645-49
GAAGAGC4960550.014.6822635
TCGCCGT939700.014.35827840-44
GTCGCCG1032350.013.89819840-44
GCCGTAT1082400.013.22871145-49
CCGTATC1183700.012.15049845-49
GGTCGCC1211150.011.96852140-44
TGGTCGC1217400.011.84396440-44
CGTATCA1259200.011.48183745-49
GTGGTCG1328150.010.93383340-44
GCGTCGT1316550.010.73007810-14
CGTCGTG1348600.010.51054910-14
GGAAGAG8163100.09.2791194
Produced by FastQC (version 0.11.5)