FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207171702
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG428650.041.9376839
GAGCGTC640800.028.4153398
ATCGGAA699000.027.4803071
TCGGAAG737700.026.1156622
CGGAAGA837150.023.2571413
AGAGCGT800350.023.0224367
AAGAGCG969650.019.937186
CGCCGTA252500.014.63550155-59
TCGCCGT291750.012.45286755-59
GCCGTAT368450.010.30519355-59
TCCGATC151300.010.1566513
CCGTATC412350.09.04282655-59
GTCGCCG426900.09.04026355-59
GAAGAGC2326100.08.834515
CGTATCA460450.08.34686460-64
TGGTCGC496650.07.83192750-54
CCGATCT201200.07.817894
CGTCGTG505850.07.675431710-14
GTGGTCG524350.07.520488350-54
TCGTGTA566550.06.924741710-14
Produced by FastQC (version 0.11.5)