FastQCFastQC Report
Thu 16 Mar 2023
EGAF00003249816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48993811
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG24164284.9321086698072945TruSeq Adapter, Index 27 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCCCGTATG799870.16325939617148785TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)