FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465042641
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG161138103.465017738018566TruSeq Adapter, Index 27 (97% over 38bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG5228110.11242216388496727TruSeq Adapter, Index 27 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA33524550.078.908471
CGGAAGA33712350.078.673753
TCGGAAG33868000.078.384362
AGCACAC33756100.078.328489
AGAGCAC34048350.077.74337
GAGCACA34318100.077.079338
GAAGAGC35660800.074.3313755
AAGAGCA37355800.070.959096
GGAAGAG39476300.067.224464
CTCGTAT22019400.023.17433440-44
TCTCGTA22152800.022.99473640-44
TATGCCG23323400.022.77784545-49
ATGCCGT23315750.022.73004345-49
ATCTCGT22550550.022.51258940-44
TGCCGTC23542300.022.38298445-49
CGTCTTC23702200.022.34279650-54
TCGTATG22799450.022.2682240-44
GCCGTCT23471300.022.12769150-54
CGTATGC23836300.021.88808645-49
CCGTCTT24315550.021.72805250-54
Produced by FastQC (version 0.11.5)