FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324144611
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC13976350.43117638010030035Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7787500.027.6477459
GAGCGTC8298300.025.9688018
AGAGCGT8812600.024.5013737
AAGAGCG9510250.022.7577926
CGGAAGA9581400.022.5692543
TCGGAAG9629500.022.5062272
ATCGGAA9638200.022.4276371
GAAGAGC11484050.019.0311955
GGAAGAG14523550.015.2184184
CGCCGTA3869900.011.39179355-59
TCGCCGT3974750.011.12119555-59
GCCGTAT4007450.011.004765555-59
CGTATCA4110700.010.79055660-64
CCGTATC4093550.010.71876555-59
GTCGCCG4220150.010.49751755-59
TGGTCGC4479450.09.9086150-54
GGTCGCC4502350.09.78383550-54
GTGGTCG4584100.09.75830650-54
TCTCGGT4925650.09.17372345-49
TCGGTGG4938950.09.03160845-49
Produced by FastQC (version 0.11.5)