FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405339183
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG4122010.10169285805265955TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1644700.044.4517331
TCGGAAG1765400.041.3985522
CGGAAGA1952650.037.3991283
AGAGCAC4226700.017.8624557
TATGCCG881950.017.37887245-49
AGCACAC4470850.017.024789
GAGCACA4581500.016.5693178
GAAGAGC4811900.015.81059555
CGTATGC1001550.015.26159145-49
CTCGTAT942000.014.81118840-44
TCTCGTA987600.014.1728340-44
ATGCCGT1127200.013.69028645-49
TATCTCG1046600.013.443130540-44
TGCCGTC1096850.013.42268645-49
GCCGTCT1140050.013.40714750-54
TCGTATG1078250.012.56311140-44
ATCTCGT1196150.011.93572140-44
GTCACGT1369400.011.46759425-29
AGTCACG1375000.011.37344425-29
CGTCTTC1400800.011.12367750-54
Produced by FastQC (version 0.11.5)