FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405324702
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG6804070.16786714371037767TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2101850.059.5056041
TCGGAAG2210500.056.5411382
CGGAAGA2396750.052.1870353
AGAGCAC4630350.027.452937
AGCACAC4871600.026.0206859
GAGCACA4996250.025.4322648
GAAGAGC5258700.024.3255845
ACGTCCG1219450.020.8200830-34
TATGCCG1245500.020.52988645-49
CGTCCGC1274000.019.96047230-34
GTCCGCA1363150.018.68483730-34
CGTATGC1365300.018.66793445-49
CTCGTAT1259750.018.1002540-44
TCCGCAC1393550.017.72999630-34
TCTCGTA1296350.017.46731840-44
AAGAGCA7563900.017.1985026
ATGCCGT1491200.017.1696145-49
TGCCGTC1461700.016.92296445-49
GCCGTCT1496700.016.8642750-54
CGCACAT1549600.016.40652335-39
Produced by FastQC (version 0.11.5)