FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405324702
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT6542630.1614170063585219Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1616500.068.7335749
GAGCGTC1909850.058.260098
ATCGGAA2257050.052.9422531
AGAGCGT2194200.050.9119767
TCGGAAG2383350.050.038972
CGGAAGA2581250.046.178253
AAGAGCG2601250.045.232486
GAAGAGC5389500.022.3251465
CGCCGTA1029800.021.5770945-49
GTCGCCG1200800.019.39807940-44
TCGCCGT1107650.019.29636640-44
AGCGGCG450650.019.2735429
GCCGTAT1269700.017.79372445-49
GGTCGCC1358450.017.29632640-44
TGGTCGC1344350.017.19301840-44
CCGTATC1368750.016.58128445-49
GTGGTCG1438850.016.11622240-44
CGTATCA1443950.015.83220445-49
GCGTCGT1525500.014.70087610-14
CGTCGTG1550600.014.51808310-14
Produced by FastQC (version 0.11.5)