FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405182909
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG5095100.12574814699304113TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1741200.053.2622681
TCGGAAG1843250.050.271062
CGGAAGA2031300.045.5738453
AGAGCAC4320000.021.811777
AGCACAC4548150.020.7812029
GAGCACA4692600.020.150918
GAAGAGC4874050.019.5360225
TATGCCG1006400.019.09916145-49
CGTATGC1129300.016.94229945-49
CTCGTAT1025400.016.47403140-44
TCTCGTA1062450.015.85313840-44
ATGCCGT1262050.015.32451745-49
TGCCGTC1222750.015.10548645-49
GCCGTCT1271250.015.02842150-54
TCGTATG1162600.013.99605940-44
AACGATC1445050.013.5566735-39
AAGAGCA7234850.013.4767186
ATCTCGT1269000.013.42019840-44
GTCACGT1477650.013.28495525-29
AGTCACG1486250.013.16710925-29
Produced by FastQC (version 0.11.5)