FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450958235
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG158237643.5089200666221343TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC32914200.077.757359
CGGAAGA32967600.077.5016563
TCGGAAG33081350.077.2982252
ATCGGAA33054150.077.2185441
AGAGCAC33144650.076.9773947
GAGCACA33443350.076.565278
GAAGAGC34717500.073.582985
AAGAGCA36561100.069.8811656
GGAAGAG38381700.066.659034
TATGCCG22482750.022.8702645-49
ATGCCGT22423350.022.84536645-49
CTCGTAT21481300.022.80492240-44
TCTCGTA21591750.022.66463340-44
CGTCTTC22714950.022.52996350-54
TGCCGTC22599050.022.52538945-49
GCCGTCT22504700.022.41793450-54
ATCTCGT21940000.022.2651740-44
CGTATGC23005450.022.0471845-49
CCGTCTT23258500.021.97002650-54
TCGTATG22216400.021.96388840-44
Produced by FastQC (version 0.11.5)