FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499474033
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG22347420.4474190553165353TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4186050.087.6132661
TCGGAAG4379400.084.068962
CGGAAGA4567700.080.525783
AGAGCAC7063250.052.2611667
AGCACAC7407350.049.7111669
GAGCACA7872250.046.8573468
GAAGAGC8074000.045.9243625
AAGAGCA10680200.034.9349336
GGAAGAG11557400.032.372434
CTCGTAT3243250.021.71327840-44
TCTCGTA3299000.021.35601440-44
TATGCCG3528650.020.95917745-49
CGCTCAT3509500.020.95805530-34
TATCTCG3385300.020.5417840-44
ATGCCGT3599200.020.4726145-49
GTCACCG3693200.020.01995325-29
ATCTCGT3592450.019.6765240-44
CCGCTCA3765500.019.59056330-34
CACCGCT3785100.019.29190430-34
ACCGCTC3875850.019.09188730-34
Produced by FastQC (version 0.11.5)