FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499474033
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC23223240.4649539008167017Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4400200.083.0103769
GAGCGTC5003500.073.2227258
ATCGGAA5104200.071.953521
TCGGAAG5339650.069.0153662
CGGAAGA5500400.066.821783
AGAGCGT5486900.066.765897
AAGAGCG6081650.060.4925886
GAAGAGC9162200.040.4983675
GGAAGAG13180250.028.4534844
CGCCGTA3462950.020.97983755-59
TCGCCGT3557600.020.34953955-59
GCCGTAT3682300.019.8222255-59
CCGTATC3803700.019.1125855-59
CGTATCA3893750.018.63762360-64
GTCGCCG4097600.017.8450455-59
TGGTCGC4318300.017.09229550-54
GTGGTCG4355050.016.9766950-54
TCTCGGT4571650.016.29432745-49
TCGTGTA4536300.016.19474810-14
GGTCGCC4583850.016.10084550-54
Produced by FastQC (version 0.11.5)