FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317275812
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG5771050.18189379025212296TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA7242550.012.6590123
TCGGAAG7248550.012.6361132
ATCGGAA7293650.012.5204811
AGAGCAC8134300.011.4514677
AGCACAC8225850.011.3328399
GAGCACA8435900.011.1227898
GAAGAGC8936850.010.4993095
TATGCCG3350950.010.1804333
ATGCCGT3404600.010.0685424
GCCGTCT3489200.09.8866176
TGCCGTC3501550.09.8186395
CGTCTTC3694000.09.4483338
AAGAGCA10470000.09.1148076
CCGTCTT4009850.08.7185567
GGAAGAG11215500.08.5626474
GTATGCC4325000.08.0002742
CGTATGC3586300.07.2450531
CTCGTAT3141050.05.94440140-44
TCTCGTA3195700.05.881769740-44
GTCTTCT6914100.05.51850139
Produced by FastQC (version 0.11.5)