FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317275812
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC5948160.18747599958864813Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6134850.014.847859
GAGCGTC6586200.013.8547048
AGAGCGT6982300.013.1131117
AAGAGCG7496500.012.2417176
CGGAAGA7534650.012.147783
TCGGAAG7554750.012.0780112
ATCGGAA7562450.012.00556851
GAAGAGC9366250.09.999945
GGAAGAG11867150.08.0757334
TCCGATC233750.07.00947483
CGCCGTA2832600.06.71667455-59
TCGCCGT2930750.06.581773355-59
CGTATCA3049000.06.423008460-64
GCCGTAT2962650.06.408621355-59
CCGTATC3029000.06.215108455-59
GTCGCCG3174050.06.178214655-59
GTGGTCG3444050.05.692806250-54
TGGTCGC3389700.05.68955850-54
GCGTCGG237650.05.583119145
GGTCGCC3451650.05.54501750-54
Produced by FastQC (version 0.11.5)