FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409178201
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT4586580.11209248168134939Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1397200.054.609949
GAGCGTC1712200.044.6522188
ATCGGAA2016200.039.948451
AGAGCGT2007750.038.3173147
TCGGAAG2132650.037.5176352
CGGAAGA2354650.034.063163
AAGAGCG2379900.033.438696
CGCCGTA852150.018.31703445-49
GAAGAGC5167850.015.9534065
GTCGCCG1035650.015.83807540-44
TCGCCGT943850.015.80534740-44
GCCGTAT1086150.014.644505545-49
GGTCGCC1204750.013.70409340-44
TGGTCGC1190650.013.6910140-44
CCGTATC1187600.013.53514945-49
CGTATCA1266800.012.73243145-49
GTGGTCG1295250.012.6771740-44
GCGTCGT1316800.011.92437310-14
CGTCGTG1341550.011.76274910-14
AGCGGCG442950.010.704659
Produced by FastQC (version 0.11.5)