FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477797063
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA859950.028.21061
TCGGAAG1025950.023.8347052
CGGAAGA1258800.019.545883
TATGCCG637200.08.09960245-49
CGATAGA671350.07.56012835-39
GCGATAG685550.07.54529530-34
CTCGTAT649800.07.53435340-44
TCTCGTA706900.06.977034640-44
AGAGCAC3929850.06.8521167
AGCACAC4250500.06.4085149
AGCGATA845700.06.28272730-34
ATGCCGT882600.06.148162445-49
GAGCACA4563150.06.0154928
CGTATGC889700.05.983406545-49
GAAGAGC4659000.05.97422175
ACACGTC966550.05.68444810-14
TGCCGTC993150.05.446278645-49
CGTCTGA1013800.05.4338815-19
ATCTCGT949000.05.42626940-44
GCCGTCT1045500.05.242848450-54
Produced by FastQC (version 0.11.5)