FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477797063
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1023600.023.7165559
GAGCGTC1475650.016.726318
ATCGGAA1543750.016.3598821
TCGGAAG1714150.014.786862
AGAGCGT1787100.013.969167
CGGAAGA1909050.013.28179553
AAGAGCG2207150.011.5273436
TCCGATC383900.09.9694243
CGCCGTA644900.07.86835155-59
CCGATCT526250.07.30024344
TCGCCGT770050.06.696887555-59
TTCCGAT609250.06.6065552
GCCGTAT905800.05.922123455-59
GAAGAGC5368650.05.3183245
CTTCCGA789950.05.30985261
CCGTATC1015400.05.298608355-59
GTCGCCG1035650.05.113811555-59
Produced by FastQC (version 0.11.5)