FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215659365
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA830000.041.609491
TCGGAAG878450.039.6834262
CGGAAGA984550.035.4177363
AGAGCAC2051850.017.4518387
AGCACAC2186950.016.4532939
GAGCACA2340600.015.3701078
GAAGAGC2473150.014.7368485
TATGCCG538400.012.93660845-49
CTCGTAT516150.012.50257540-44
TCTCGTA538150.011.85675540-44
ATGCCGT610250.011.42297245-49
CCGGAGA658050.010.679214530-34
TGCCGTC665350.010.42905645-49
CGTATGC682600.010.286571545-49
AAGAGCA3659850.010.1664146
ACACGTC714550.010.04905710-14
GCCGTCT680350.09.962450-54
CGTCTGA727450.09.91071415-19
ATCTCGT653250.09.87195540-44
ACTCCGG734300.09.84670630-34
Produced by FastQC (version 0.11.5)