FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215659365
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG647950.046.1294639
GAGCGTC865850.034.7800648
ATCGGAA950900.033.894981
TCGGAAG1002300.032.3587652
CGGAAGA1094150.029.7222523
AGAGCGT1035700.029.3980717
AAGAGCG1229900.026.306136
CGCCGTA369550.015.95298855-59
TCGCCGT412300.013.89797455-59
GAAGAGC2612750.012.821345
GCCGTAT486150.012.44290755-59
CCGTATC520650.011.42902955-59
AGCGGCG290350.011.0858169
GTCGCCG564100.010.84429455-59
CGTATCA578550.010.42356960-64
TGGTCGC625000.09.79690750-54
GTGGTCG639500.09.67453350-54
GAGCGGC356150.09.1394478
ATCGGGA246900.09.0263461
GGTCGCC705250.08.85481950-54
Produced by FastQC (version 0.11.5)