FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389810964
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG8345270.21408505072217518TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3626200.042.623951
TCGGAAG3725750.041.4431422
CGGAAGA3879050.039.8113143
AGAGCAC5859850.026.6787977
AGCACAC6061450.025.8464859
GAGCACA6182850.025.3448548
GAAGAGC6491800.024.202535
AAGAGCA8609300.018.461856
TATGCCG1776450.018.33574945-49
CGTACGT1933650.016.90073630-34
CGTATGC1917150.016.81614545-49
CCGTACG1951200.016.73534830-34
GGAAGAG9674200.016.544394
CTCGTAT1774000.016.33801540-44
ATGCCGT1994700.016.27502845-49
GCCGTCT1979250.016.15106650-54
TCTCGTA1829700.015.84223740-44
TGCCGTC1956650.015.48225945-49
ACCGTAC2164200.015.13716430-34
CGTAATC2200800.014.94227935-39
Produced by FastQC (version 0.11.5)