FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408875416
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1452850.037.2153971
TCGGAAG1557000.034.5789152
CGGAAGA1742050.030.9176243
TATGCCG761700.015.00696945-49
AGAGCAC4024950.013.960667
AGCACAC4289500.013.1064139
CGTATGC874350.013.03203945-49
GAGCACA4410200.012.7871578
CTCGTAT821100.012.36052240-44
GAAGAGC4629600.012.238235
TCTCGTA861550.011.7902940-44
GTCACGA999800.011.48524625-29
ATGCCGT1016900.011.37486245-49
GCCGTCT1005150.011.28588250-54
TGCCGTC972800.011.17810245-49
TATCTCG919000.011.05954840-44
ACGAGTG1153550.010.10780230-34
TCGTATG984500.09.85541840-44
ATCTCGT1055550.09.82801240-44
CACGAGT1182850.09.77284930-34
Produced by FastQC (version 0.11.5)