FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400601924
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG4714050.11767417272813697TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2172250.039.8650051
TCGGAAG2275850.037.9232862
CGGAAGA2439000.035.394923
AGAGCAC4593100.019.2178257
AGCACAC4858100.018.1815369
GAGCACA4970200.017.826248
GAAGAGC5251450.016.9868895
TATGCCG1059650.016.70397845-49
CGTATGC1177000.014.8833245-49
CTCGTAT1111700.014.29363640-44
TCTCGTA1147250.013.80016240-44
ATGCCGT1306350.013.60387445-49
TGCCGTC1274000.013.43144745-49
GCCGTCT1297650.013.42401150-54
TATCTCG1212750.012.92928440-44
TCGTATG1258800.012.3272940-44
AAGAGCA7492050.012.1408346
ATCTCGT1355550.011.80364640-44
CGTCTTC1559850.011.50497950-54
CCGTCTT1600000.011.21265150-54
Produced by FastQC (version 0.11.5)