FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400601924
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT5102400.1273683348560253Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1826500.045.3755579
GAGCGTC2153550.038.5754628
ATCGGAA2554200.034.0003431
AGAGCGT2490450.033.5113837
TCGGAAG2656650.032.462612
CGGAAGA2846850.030.2218133
AAGAGCG2902300.029.4316466
GAAGAGC5549200.015.8592455
CGCCGTA1051850.015.85357345-49
TCGCCGT1143650.014.25028740-44
GTCGCCG1246150.013.87394140-44
GCCGTAT1282900.013.23340745-49
CCGTATC1377650.012.37587745-49
GGTCGCC1422600.012.19794940-44
TGGTCGC1430950.012.10346940-44
CGTATCA1458150.011.80897345-49
GTGGTCG1547750.011.32872240-44
GGAAGAG8815250.010.4096854
GCGTCGT1716850.09.76513210-14
CGTCGTG1744000.09.65551210-14
Produced by FastQC (version 0.11.5)