FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404944670
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1116900.049.096751
TCGGAAG1270400.043.4930272
CGGAAGA1496250.037.0103763
CGCGAAA593850.018.26345635-39
TCCGCGA614100.017.84516530-34
CCGCGAA638200.017.10767430-34
AGAGCAC3716400.015.4329967
AGCACAC4010100.014.2773979
GAGCACA4227450.013.6513448
TATGCCG839200.013.45387945-49
GAAGAGC4338600.013.3518085
CTCGTAT836350.012.85168940-44
CTCCGCG873400.012.829411530-34
GCGAAAT912850.012.31642135-39
TCTCGTA879200.012.17750440-44
ACTCCGC995900.011.34742930-34
ATGCCGT1021600.011.16105945-49
CGTATGC1053150.010.77162945-49
ATCTCGT1092300.010.0128240-44
TGCCGTC1148350.09.90389745-49
Produced by FastQC (version 0.11.5)