FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404944670
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1205750.045.032319
GAGCGTC1603400.034.098688
ATCGGAA1624750.034.042491
TCGGAAG1796700.030.9807592
AGAGCGT1865750.029.5259747
CGGAAGA1999550.027.8116653
AAGAGCG2228800.025.0120056
CGCCGTA762750.014.37639955-59
TCGCCGT873850.012.61663255-59
GAAGAGC4898600.011.9192275
GCCGTAT989700.011.25405455-59
CCGTATC1101100.010.15628355-59
GTCGCCG1100400.010.14166355-59
CGTATCA1163100.09.67365760-64
TGGTCGC1231500.09.24085250-54
GTGGTCG1300550.08.8360750-54
CGTCGTG1313950.08.4666910-14
GGTCGCC1357650.08.42279450-54
GCGTCGT1397050.08.01703610-14
CCGTGTA1457100.07.897464335-39
Produced by FastQC (version 0.11.5)