FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483133214
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG37122840.7683768973912856TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA10667500.057.9146841
TCGGAAG10783100.057.4899982
CGGAAGA10838400.057.106333
AGAGCAC12618100.049.2017177
AGCACAC12820600.048.421199
GAGCACA13206550.047.0110638
GAAGAGC13784400.045.1780555
AAGAGCA16032750.038.914386
GGAAGAG17232100.036.2171364
CTCGTAT6277900.018.82581940-44
TATGCCG6658650.018.66528745-49
TCTCGTA6357350.018.57002440-44
ATGCCGT6742250.018.38374945-49
ATCTCGT6702400.017.61487440-44
TGCCGTC7020550.017.54555945-49
GCCGTCT7023150.017.37794150-54
CGTATGC7129400.017.2323345-49
CCGGAGA7318650.016.90244930-34
CGTCTTC7433600.016.77025650-54
TCGTATG7127750.016.54743240-44
Produced by FastQC (version 0.11.5)