FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483133214
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC39126800.8098553124935848Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG9982350.061.7678879
GAGCGTC10646450.057.9463848
AGAGCGT11265850.054.6217467
ATCGGAA11524350.053.6023251
TCGGAAG11706550.052.927262
CGGAAGA11767300.052.6154673
AAGAGCG12058600.051.2614866
GAAGAGC14899650.041.6981245
GGAAGAG18833800.033.212584
CGCCGTA6793950.018.22761355-59
TCGCCGT6914900.017.85344355-59
GCCGTAT6960150.017.80027655-59
CCGTATC7074600.017.44058655-59
CGTATCA7095600.017.3799360-64
GTCGCCG7457750.016.65886155-59
TGGTCGC7729050.016.1712150-54
GTGGTCG7854650.015.97002450-54
GGTCGCC7914500.015.77543850-54
TCTCGGT8236900.015.32952745-49
TCGGTGG8309500.015.1158645-49
Produced by FastQC (version 0.11.5)