FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003249886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974563
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGAC38770.39781933030496747No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA37470.3844800182235525No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG34620.35523614173737356No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT34510.3541074307151No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA31390.32209308171970413No Hit
CTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAG27140.27848379222277064No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA27050.27756030138636495No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC23480.24092849820894083No Hit
CACTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACG23250.2385684660714597No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC20800.21342899330263923No Hit
GGGCATTTTCACCTTGGGCATCTTCAGGTGCCAGTCTGGGCCATGAACAT20220.2074776079124695No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC20090.20614367670432798No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA20000.20522018586792232No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA19890.20409147484564877No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC18770.19259914443704512No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC18680.19167565360063946No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG18490.18972606183489418No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT17200.17648935984641323No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC14940.153299478843338No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG14800.15186293754226254No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG14790.15176032744932857No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA14650.15032378614825312No Hit
GTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCAAAGA14550.1492976852189135No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA14540.14919507512597954No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT14130.14498806131568714No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG13990.1435515200146117No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG13680.14037060713365887No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT13460.13811318508911175No Hit
CCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAA13400.137497524531508No Hit
CTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTT13360.1370870841597721No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG13200.13544532267282874No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC13010.13349573090708347No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC12930.13267485016361177No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT12760.13093047858373444No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC12600.12928871709679107No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG12500.12826261616745147No Hit
GTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGT12380.12703129505224392No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC11890.12200340049847982No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT11560.11861726743165911No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC11520.11820682705992328No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA11440.11738594631645158No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT11360.11656506557297988No Hit
CTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAAC11170.11461547380723462No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG11050.11338415269202709No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA10970.11256327194855542No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC10810.11092151046161203No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT10800.11081890036867806No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT10790.11071629027574409No Hit
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTAAGAC10720.10999801962520639No Hit
GTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACG10670.10948496916053656No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC10560.108356258138263No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG10230.10497012507144228No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT10230.10497012507144228No Hit
GGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCA10020.1028153131198291No Hit
GATGGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGA9760.1001474507035461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)