FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003249887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974563
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTCTGACTACAACATCCAGAAAGAGTCTACCCTGCACCTGGTGCTC45980.4718012073103534No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC43760.44902176667901406No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29630.30403370536332697No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC28210.28946307216670447No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC27260.27971511333797816No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA23910.24534073220510116No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT23700.24318592025348798No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA22390.22974399807913903No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA20800.21342899330263923No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC20700.21240289237329962No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG20690.21230028228036565No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC19710.20224449317283746No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC19200.19701137843320546No Hit
CTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACA18300.18777647006914894No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA17310.17761807086868678No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT17270.17720763049695093No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC17050.1749502084524038No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC16980.17423193780186608No Hit
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC16340.16766489185409256No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC16280.1670492312964888No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC16140.16561268999541334No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG16050.16468919915900768No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT15810.1622265569285926No Hit
GATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACC14860.1524785980998663No Hit
CAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGG14570.14950290540478142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14500.1487846347542437No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC14290.1466298228026305No Hit
CCTGTCCCTGGCCGACAAGGATGTGACTGCCAAAGACAGCAAGTTCAAAA13970.14334629982874375No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC13830.1419097585276683No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT13690.14047321722659284No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC13600.13954972639018717No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT13550.13903667592551738No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT13480.13831840527497966No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT13380.13729230434564005No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC13310.13657403369510232No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA13230.13575315295163065No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA13210.1355479327657627No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA12920.13257224007067783No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA12600.12928871709679107No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA12360.126826074866376No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC12250.12569736384410243No Hit
CTACAACATCCAGAAAGAGTCCACCTTGCACCTGGTGCTCCGTCTCAGAG11740.12046424910447041No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT11530.11830943715285722No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC11470.11769377659525346No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC11400.11697550594471573No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT11350.11646245548004593No Hit
TGGAGGTGGACGTCCAGGCCCCGAGAGCAAAGCTGGATGGTGCGCGGCTG11320.11615462520124405No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA11270.11564157473657424No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT11230.1152311343648384No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC11140.11430764352843276No Hit
CCTCAAGGGTCCTGAAGTTGACATCAAGGGCCCCAAAGTGGACATTAATG11090.11379459306376295No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG11040.11328154259909312No Hit
CACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGC11000.11287110222735729No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA10910.11194761139095165No Hit
AGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTG10770.11051107008987618No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC10500.10774059758065922No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT10420.10691971683718755No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC10410.10681710674425358No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG10260.10527795535024416No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC10060.10322575349156493No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC10020.1028153131198291No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTCAGAG9880.10137877181875363No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAG9840.10096833144701779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)