FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003249891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332370
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN622974.6756531594076725No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT31170.233944024557743No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC29700.22291105323596297No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC28350.2127787326343283No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC28300.21240346150093442No Hit
CGACAAGGTGCGGTTCCTGGAGCAGCAGAACAAGGTTCTGGAAACAAAGT26590.1995691887388638No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC26320.1975427246185369No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA25870.194165284417992No Hit
CTACAACATCCAGAAAGAGTCTACCCTGCACCTGGTGCTCCGTCTCAGAG25720.19303947101781035No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT25680.19273925411109527No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC25600.19213882029766507No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC24530.1841080180430361No Hit
TGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTG24130.18110584897588508No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT23890.17930454753559447No Hit
CCTGTCCCTGGCCGACAAGGATGTGACTGCCAAAGACAGCAAGTTCAAAA23130.17360042630800754No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC23090.17330020940129243No Hit
GGATGTGTCTGTGCCCAAGGTAGAAGGTGAAATGAAAGTGCCAGATGTTG22600.16962255229403242No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG21770.16339305147969407No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA21440.16091626199929449No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC21270.15964034014575532No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT21220.15926506901236143No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC21140.15866463519893123No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG20550.15423643582488347No Hit
CTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGA20510.1539362189181684No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC20450.15348589355809572No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG20350.15273535129130797No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC18940.14215270532960064No Hit
CCGAGGAGCGCGAGCAGATCAAGACCCTCAACAATAAGTTTGCCTCCTTC18810.14117700038277656No Hit
CTTTGCCGGAAAGCAGCTGGAAGATGGGCGCACCCTGTCTGACTACAACA18400.13809977708894677No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC17980.1349474995684382No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA17970.1348724453417594No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC17970.1348724453417594No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC17880.13419695730165043No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT17770.1333713608081839No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG17750.13322125235482637No Hit
CTCCTTCATCGACAAGGTGCGGTTCCTGGAGCAGCAGAACAAGGTTCTGG17570.1318702762746084No Hit
CCTGGAGCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGCAGCTGG17350.13021908328767534No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT16980.12744207690056067No Hit
CCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAG16970.1273670226738819No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA16460.12353925711326434No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT16280.12218828103304638No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC16200.12158784721961619No Hit
CCTGAAGATGCCCAAGGTGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA15920.11948632887261046No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGC15910.11941127464593168No Hit
AGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTG15620.11723470207224719No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT14890.1117557435246966No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT14580.10942906249765455No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC14420.10822819487079414No Hit
TGGATGTGTCTGTGCCCAAGGTAGAAGGTGAAATGAAAGTGCCAGATGTT13830.1037999954967464No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA13410.10064771797623784No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)