FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003249901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301756
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA39710.17252045829358106No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT38330.1665250356684201No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT31270.13585280107882852No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT30950.13446255815125496No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC29990.13029182936853428No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC29560.1284236904346073No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC26420.1147819317077918No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC25520.11087187347399116No Hit
CGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCAC25200.1094816305464176No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT24010.10431166465950344No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT23580.10244352572557647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)