FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335732673
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG32586650.9706130091187162TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC14740150.035.4207239
AGAGCAC14798400.035.2930567
GAGCACA14919000.034.99618
CGGAAGA14957550.034.9452823
TCGGAAG15091250.034.6243322
ATCGGAA15330500.033.9900931
GAAGAGC15943750.032.8022165
AAGAGCA17368700.030.1549266
GGAAGAG18324700.028.6854844
CTCGTAT6542900.015.65272340-44
TCTCGTA6639100.015.40276440-44
ATCTCGT6936100.014.67298540-44
TATGCCG7226900.014.62474645-49
TCGTATG6994350.014.58602840-44
ATGCCGT7292600.014.51267545-49
TGCCGTC7336300.014.28826945-49
CGTCTTC7435350.014.17689250-54
GCCGTCT7284500.014.1456250-54
CGTATGC7515100.013.76579345-49
CCGTCTT7816300.013.44661250-54
Produced by FastQC (version 0.11.5)