FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335732673
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC33556740.9995077244090569Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG12605050.041.158949
GAGCGTC13220400.039.2786648
AGAGCGT13895600.037.4441157
AAGAGCG14792800.035.3073466
CGGAAGA15321700.034.1940043
TCGGAAG15482250.033.8442842
ATCGGAA15701650.033.268261
GAAGAGC16523950.031.7282055
GGAAGAG19454900.027.0920564
CGCCGTA6107050.017.15186555-59
GCCGTAT6223000.016.85603755-59
TCGCCGT6221200.016.84484155-59
CGTATCA6341050.016.51646860-64
CCGTATC6320400.016.47842455-59
GTCGCCG6491300.016.13388855-59
TGGTCGC6718150.015.57208250-54
GGTCGCC6772550.015.385850-54
GTGGTCG6847700.015.34117850-54
TCTCGGT7311350.014.50012645-49
TCGGTGG7244600.014.446345-49
Produced by FastQC (version 0.11.5)