FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003249925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256897
Sequences flagged as poor quality0
Sequence length126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC53680.23784869225312455No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT52980.2347470885911054No Hit
CTTCTTCAATCAGCACCACTGAGACCACATCCCACAGTACTCCCAGCTTC48870.21653624423267878No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA47280.20949117305752102No Hit
CCACCACCGACTCGATCGTCACCACCGAGACCACATCCCACAGTACTCCC39700.1759052362602281No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC34370.15228873980513954No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC32170.14254084258165084No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT30640.13576162314895185No Hit
CTTCTTCGATCACCACCACCGAGACCACATCCCACAGTACTCCCAGCTTC30280.13416651269419916No Hit
CCTCACACAATACTCCCAGCTTCACTTCTTCGATCACCACCACCGAGACC30260.1340778954467129No Hit
CTTCTTCAATCAGGACCACTGAGACCACCTCTCATAGTACTCCCAGCTTC28630.12685558977658262No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC26750.11852556851287409No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC26010.11524673035588243No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT25760.11413901476230418No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC24260.1074927212008346No Hit
CCACCACCGAGACCCCCTCAAGCAGTACTCCCAGCTTCACTTCTTCGATC23840.10563175900362312No Hit
CCCAGCTTCACTTCTTTGATCACCACCACGGAGACCACCTCACACAGTAC23470.10399233992512728No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT23420.10377079680641164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)