FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003249929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640481
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC54990.3352065644161682No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC42810.260960047693329No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC36610.22316625428761444No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA36000.2194478326783425No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTG33240.20262349883966957No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC31730.1934188814134391No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT31200.19018812165456353No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG30610.18659161550789066No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT28010.17074260537001038No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA27550.16793854973023156No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT27320.16653652191034213No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG27270.16623173325384444No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG26700.1627571425697707No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG25910.15794148179710707No Hit
CATGGATGAAATTGAAAAGTACAAAGAAGTGGAAGAAGACCAAGACCCAT25140.15324773648704254No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG25090.15294294783054482No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG24820.15129708908545725No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC24650.15026080765336508No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT24030.14648142831279362No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC23920.1458108932684987No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA23640.14410407679211157No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC23500.14325066855391805No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT23340.1422753448531254No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC22880.1394712892133466No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC22180.1352042480223788No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAG21640.13191253053220367No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC21610.13172965733830505No Hit
CTTTGCCGGAAAACAGCTGGAAGATGGTCGTACCCTGTCTGACTACAACA21070.12843793984812993No Hit
CTACAACATCCAGAAAGAGTCCACCTTGCACCTGGTGCTCCGTCTCAGAG21040.12825506665423128No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC20930.12758453160993635No Hit
ACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACT20610.1256338842083511No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT20290.12368323680676582No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA20200.12313461722506998No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT20170.12295174403117133No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC20040.12215929352427733No Hit
GTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTG19590.11941619561579805No Hit
TGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTG19070.11624639358822199No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT18890.11514915442483029No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA18380.11204031012855376No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA17770.10832188851928184No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAG16930.10320143909012053No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT16650.10149462261373342No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC16610.10125079168853525No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA16500.10058025664424032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)