FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003249931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5821099
Sequences flagged as poor quality0
Sequence length101-126
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA191850.32957694071171095No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC186180.31983651197136487No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG165770.2847744042834523No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT161400.2772672308098522No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT149730.25721947006913987No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC146260.2512583963955947No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA142660.24507399719537495No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA139410.23949085902850992No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT135420.23263648324826636No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT134190.23052348018819127No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT131290.2255416030546809No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC130850.2247857320413207No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC123920.2128807635808977No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC120750.20743505650737087No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG119460.20521898012729212No Hit
ACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCT117820.20240164271385866No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT117560.20195499166050945No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT115280.19803820550037027No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC106750.1833846151731829No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC104340.17924450348636914No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT103730.17819659139966526No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG98990.1700537991193759No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTG97970.16830155267931363No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC95340.16378350548581977No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA95010.16321660222579962No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC93430.16050233813236983No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT93230.1601587603990243No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAG89400.1535792468054572No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG89360.15351053125878805No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG89160.15316695352544252No Hit
GATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA84590.1453162023184969No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG83990.14428546911846027No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA81690.14033432518498654No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT81690.14033432518498654No Hit
CAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGG80040.13749980888488583No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC78990.13569602578482173No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA77330.13284433059805373No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA76870.132054101811359No Hit
AGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG75990.1305423597846386No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA75640.1299410987512839No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA75290.1293398377179292No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC74370.12775938014453972No Hit
TTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGG73530.12631635366448846No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT73470.12621328034448479No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT73450.1261789225711502No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG73280.1258868814978065No Hit
AGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCA72020.1237223417777296No Hit
GCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT71350.12257135637102204No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGC70960.12190137979099823No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC70650.12136883430431264No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC70600.12128293987097626No Hit
GGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGAT68280.11729743816416796No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC68250.11724590150416614No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG67620.11616363164412769No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTG67310.1156310861574421No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA67030.11515007733075833No Hit
CATGGATGAAATTGAAAAGTACAAAGAAGTGGAAGAAGACCAAGACCCAT66850.11484085737074735No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA66400.11406780747071987No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT66130.11360397753070339No Hit
GCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTG65790.11301989538401597No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC65510.11253888655733221No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA65480.11248734989733039No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG63880.10973872803056604No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG62300.10702446393713626No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC61670.10594219407709782No Hit
CCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCT60950.10470531423705386No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC60920.10465377757705202No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCA60770.10439609427704288No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC60460.10386354879035728No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAG60450.10384636990369No Hit
CATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTC59890.1028843522503225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)