FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003249933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367232
Sequences flagged as poor quality0
Sequence length101-126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50980.37287014932359686No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA45540.3330817300940879No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT41270.30185074661798433No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC27950.2044276318869073No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC27100.19821069138229647No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT24930.1823392079764078No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA23920.17495201984739972No Hit
CTTCTTCAATCAGCACCACTGAGACCACATCCCACAGTACTCCCAGCTTC22280.16295698169732717No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG22190.16229871740860366No Hit
TCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTT20520.1500842578289566No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC19420.14203880541122502No Hit
CTTCTTCGATCACCACCACCGAGACCACATCCCACAGTACTCCCAGCTTC19030.13918632682675655No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT18810.13757723634321023No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC18410.13465161728221692No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC17460.12770327201235782No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT17160.12550905771661283No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA16680.12199831484342087No Hit
CCACAACAGCCACTACGACTGAGTCCACTGGATCCACGGCCACCCCGTCC15940.11658591958058324No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT15930.11651277910405841No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC15790.11548881243271077No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC15200.11117352431774565No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT15010.10978385526377382No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC14750.10788220287412816No Hit
GTTCAAGATGTGCTTCAACTATGAAATCCGTGTGTTCTGCTGCAACTACG14430.10554170762533352No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC14050.10276236951738987No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT13990.10232352665824089No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC13840.1012264195103684No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC13750.1005681552216449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)