FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003249938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145008
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG75980.6635761496862904No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC69820.609777398935204No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA64540.5636641840057013No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG63940.5584240459455305No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA60120.5250618336291101No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG55790.4872455039615444No Hit
GGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCAT54280.4740578231767813No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA50440.4405209395916885No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG46760.4083814261559744No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT38300.33449547950756675No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT31810.27781465282338635No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT30630.26750904797171726No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT30280.26445230076995097No Hit
GAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATC30230.26401562259827005No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG29890.26104621103083997No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG27310.2385136173721057No Hit
GAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGT27030.2360682196106927No Hit
CCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTG26870.2346708494613138No Hit
TCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCG26860.23458351382697765No Hit
GGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAACAGCC26740.23353548621494347No Hit
CTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAAT26450.2310027528191943No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG26040.2274219918114109No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG25650.22401590207229996No Hit
GTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGA25510.2227932031915934No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA25160.21973645598982713No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT24320.2124002627055881No Hit
AGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTT24110.21056621438452833No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT23740.2073347959140897No Hit
GTAGGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAAT23060.2013959727792295No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC22700.19825188994312704No Hit
GGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTAGTTC22640.19772787613710996No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA22570.1971165266967567No Hit
GGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTC22100.19301175188295627No Hit
CCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAG22010.19222573117393066No Hit
CATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTG22000.1921383955395945No Hit
TTCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG21610.18873230580048347No Hit
TCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC21530.18803362072579408No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC21210.18523888042703632No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA20500.1790380503891676No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT20190.17633064572474602No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC19820.1730992272543074No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT19770.1726625490826265No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC19650.17161452147059233No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC19430.1696931375151964No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC19360.16908178807484314No Hit
CTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGT18710.1634049718429915No Hit
GGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATC18680.16314296493998295No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA18150.15851417632016543No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG18130.15833950505149308No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC17960.15685479926777804No Hit
GGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTT17770.15519542221539062No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA17250.1506539692299093No Hit
CTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCA17220.15039196232690077No Hit
CGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT17120.14951860598353897No Hit
GTCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC17110.14943127034920278No Hit
CTCTGGTTATATTTGAAAAAGAATCTTTCTCATCACTTGTAGCCTTCAAG17070.14908192781185808No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG17030.14873258527451336No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT16930.14785922893115155No Hit
TCCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTG16890.14750988639380685No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG16880.14742255075947067No Hit
GCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTT16700.14585050934141944No Hit
ATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGA16480.1439291253860235No Hit
CCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTTGTACTTTTCAA16200.1414837276246105No Hit
GACCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG16190.1413963919902743No Hit
CTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAA16140.1409597138185934No Hit
GGTTGCTCAGAAGACACTGTCCTAGATTTTTCTCCATCCTTTTCTTCTCT16070.14034836437824016No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT15760.1376409597138186No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC15690.13702961027346536No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC15590.13615625393010355No Hit
GCCCATAGGTCCTGCCTGCGGCAATGACATCTCTCGGGTCAGTAAGGGCC15520.1355449044897503No Hit
GCTCCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCT15440.13484621941506084No Hit
TGTCCGCGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACA15430.13475888378072468No Hit
GGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGG15430.13475888378072468No Hit
GGCGCTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG15380.1343222056090438No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT15360.13414753434037144No Hit
CCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCGAGAG15260.13327417799700964No Hit
GCTTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT15050.13144012967594987No Hit
AAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGA14900.13013009516090718No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC14760.12890739628020068No Hit
TGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAA14750.1288200606458645No Hit
GCTCTACTACCTCCAGCAGCTCCCTGCTGAGCCTGGGGCATGGTGGGCCT14580.12733535486214945No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAACCAAGCCAAGGTACTGTTCCT14480.12646199851878764No Hit
GTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGC14470.12637466288445148No Hit
GTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAGA14140.12349258695135755No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC14050.12270656624233194No Hit
CTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTA14020.12244455933932338No Hit
GAGGTACTCACTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCA14010.12235722370498722No Hit
CCCTGCTGAGCCTGGAGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG13960.12192054553330632No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCGAGAGAAAAGCCAACATGTTTTT13640.11912580523454858No Hit
CCCGGGCACAAGCAGGTAGATCATCACTCTAAGGACACCACCCTCATCCA13540.11825244889118679No Hit
AATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG13370.11676774310747173No Hit
GCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGG13220.11545770859242904No Hit
GCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGTCC13070.11414767407738637No Hit
GCTTCCTCTTCTTGGTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCC12980.11336165336836075No Hit
CCTCCCTGTGGCAATGACATCTCTCAGCTCAGTAAGGGCCACTTGCAGTA12870.11240096139066279No Hit
AGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTT12800.11178961195030951No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC12740.11126559814429245No Hit
CCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT12650.11047957743526683No Hit
GAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATCATC12650.11047957743526683No Hit
GTCTGGTCCACCTACAGTAGGTTAGTAAATGATAAGGGGAGGAAGAAATG12650.11047957743526683No Hit
AGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCA12600.11004289926358592No Hit
CATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAG12580.10986822799491357No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG12500.10916954292022414No Hit
AGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGT12420.1084708578455347No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA12400.10829618657686234No Hit
ACCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTC12360.10794684403951764No Hit
CCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGT12350.10785950840518145No Hit
GGTGGGTCTTGGTCTTCTTCCTCTTCTTCGTCCTTTTTAATTCTGTCCAC12330.10768483713650909No Hit
CATCCATGTCAACAGCCAAGCCAACACACTGTTGCTCCAATATGTAAAAG12270.10716082333049202No Hit
TCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTC12140.10602546008412168No Hit
CCGCCTCTTGGGTTCGGGTGATGATGGTTCCACGTCAGCGGCCCTCCGCC12060.10532677500943224No Hit
GCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAA11960.10445341866607047No Hit
GTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTC11920.10410407612872574No Hit
CGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCT11890.10384206922571719No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGATGCTCGGAAGGTGTCTTG11790.10296871288235541No Hit
GGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTT11790.10296871288235541No Hit
CCCCTGCTGAGCCTGGGACCTGTTGCCTCTTGGTCCTCCTTTTTCACTTG11690.1020953565389936No Hit
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTAAGAC11680.10200802090465744No Hit
GGCCCGATGCAGCTTTTGCTGAAATCCAGCGGACTTCTCTCCCTGAGAAG11610.10139667146430419No Hit
GCAGGAATATGATCTTTATATGGAAGACTCAGTGGATGCTTATCACCTTC11590.10122200019563182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)