FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003249939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145008
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT51780.4522239145927365No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT49470.432049383061079No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC49270.43030267037435543No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT42030.36707167111496164No Hit
ACAGCGTGTTGGCTTGGCTGTTGACATGGATGAAATTGAAAAGTACCAAG39740.3470718108519766No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC37840.3304780403281025No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC36980.32296717577519113No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA34090.2977271774520353No Hit
ACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGAC33770.2949324371532775No Hit
TCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTT33000.2882075933093917No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC31450.27467056998728395No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA30860.26951776756144935No Hit
AGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCAC29980.2618322317398656No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA29740.2597361765157973No Hit
GCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTG29220.25519472353031597No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT27780.2426183921859061No Hit
CAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGG27650.24148302893953583No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA27310.2385136173721057No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC27300.23842628173776953No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC26310.22978005393848774No Hit
CTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGG26090.2278586699830918No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA25770.22506392968433409No Hit
GCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGG25500.22270586755725724No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC24880.2172910582284141No Hit
CTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTGTTGGCTTGGCTG24340.21257493397426044No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA24190.21126489945921778No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA24190.21126489945921778No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA23720.20716012464541733No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT23410.20445271998099576No Hit
CACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCA22960.2005226164358677No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT22760.19877590374914408No Hit
TAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAG22710.1983392255774632No Hit
GCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCG22580.19720386233109286No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT22030.192400402442603No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG21390.18681092184508755No Hit
CTTTACTTTTTCCCACTTTTCCAGGCTCAGCAGGGAGCTGCTGGATGAGA21240.18550088733004486No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG20880.1823568044939424No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC20550.17947472856084848No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC20550.17947472856084848No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA20170.17615597445607367No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG19930.17405991923200537No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC19770.1726625490826265No Hit
GGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGAT19750.17248787781395414No Hit
GCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATA19680.17187652837360087No Hit
GATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACC19490.17021715132121348No Hit
AGAAACATCTAGGACAGTGTCTTCTCAGAAACCACCAGCCTTGAAGGCTA19100.1668110615821025No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG18350.16026088900688903No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC18280.15964953956653577No Hit
CCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGGT18150.15851417632016543No Hit
GTCCTTTTTCTTTTCAAACTCTTCCTTACGTTAGCCATGAAATCTAGCTG18110.15816483378282073No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT17590.1536233807973394No Hit
CGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATG17410.1520513393792882No Hit
CTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTG17260.15074130486424547No Hit
CTTCCACCCTCAGCAGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT17200.15021729105822842No Hit
CATGGATGAAATTGAAAAGTACAAAGAAGTGGAAGAAGACCAAGACCCAT16980.14829590710283247No Hit
TTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTA16690.14576317370708328No Hit
GAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTC16550.14454047482637675No Hit
CAGGGAGCTGGTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG16500.14410379665469586No Hit
TGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCC16220.14165839889328283No Hit
ACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACT16210.14157106325894667No Hit
CTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAAAACATG16010.1398243505722231No Hit
GAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA15910.13895099422886129No Hit
CCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACC15880.13868898732585275No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC15870.1386016516915166No Hit
TTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAG15800.13799030225116332No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA15700.13711694590780152No Hit
CAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTG15690.13702961027346536No Hit
CTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTC15660.1367676033704568No Hit
CAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGA15620.13641826083311206No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT15490.13528289758674175No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG15430.13475888378072468No Hit
TGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACA15380.1343222056090438No Hit
CTCAAGACACCTCCCGAGTGTCTGCTCACTCCCCTTCCACCCTCAGCGGA15350.13406019870603525No Hit
CGGATGATAATCTCAAGACACCTCCCGAGTGTGTGCTCACTCCCCTTCCA15240.13309950672833726No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC15200.13275016419099253No Hit
CCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCG15100.13187680784763076No Hit
CTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATTGGAGCA15080.1317021365789584No Hit
CTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCC14990.1309161158699328No Hit
GAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCC14830.1295187457205539No Hit
GCCAGCCCTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTGTTGGC14680.12820871120551122No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT14550.12707334795914088No Hit
GAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATTGGA14480.12646199851878764No Hit
AGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG14420.12593798471277057No Hit
AGAAGTGCCTTTTACGTATTGGAGCAACAGCGTGTTGGCTTGGCTGTTGA14260.12454061456339169No Hit
CAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTG14220.12419127202604698No Hit
TGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTG14180.12384192948870228No Hit
ACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTGTTGGCTTGGCTGTT14150.12357992258569372No Hit
TCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCAT14040.12261923060799575No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA14010.12235722370498722No Hit
TGGCTGGGCTCTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAG13990.12218255243631486No Hit
TCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCA13970.1220078811676425No Hit
TGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCA13920.12157120299596161No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG13860.12104718918994452No Hit
AGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTC13800.12052317538392744No Hit
CACCTTCCGAGCGTCAGCTCACTCCCTTTCCACCCTCAGCTCCACCCTCA13760.12017383284658274No Hit
CTTGGCTGTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAG13640.11912580523454858No Hit
CCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGG13630.1190384696002124No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC13620.11895113396587621No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG13560.11842712015985915No Hit
CCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCC13500.11790310635384207No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC13470.11764109945083354No Hit
CAACAGCGTGTTGGCTTGGCTGTTGACATGGATGAAATTGAAAAGTACCA13440.11737909254782498No Hit
CCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGC13400.11702975001048027No Hit
CCCCTTCCACCCTCAGCTCCACTCTCAGCAGATGATAATATCAAGATACC13230.11554504422676523No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGATTCCTTCAGGTTATCT13120.11458435224906725No Hit
CCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCA13110.11449701661473106No Hit
TGCCAGCCCTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTGTTGG13080.11423500971172254No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC13060.11406033844305018No Hit
AGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAA13000.1135363246370331No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT12840.11213895448765424No Hit
AGAAGTGCCTTTTACATATTGGAGCAACAGTGTGTTGGCTTGGCTGTTGA12760.11144026941296481No Hit
GCTCACTCCCCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTCCCG12730.11117826250995626No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT12630.11030490616659447No Hit
GTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCCC12570.10978089236057739No Hit
CGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGA12520.1093442141888965No Hit
GCTCTACCCTCAGCTCCACCCTCAGCGGATGATAATCTCAAGACACGTGC12440.10864552911420705No Hit
GCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGC12390.10820885094252616No Hit
CTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTC12390.10820885094252616No Hit
TGCTCACTCCCCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTCCC12180.1063748026214664No Hit
CCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCT12120.10585078881544933No Hit
AGAAGAGGAAGAAGACCAAGACCCACCATGCCCCAGGCTCAGCAGGGAGC12120.10585078881544933No Hit
CTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTG12110.10576345318111313No Hit
CAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTT12090.10558878191244078No Hit
CTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAA12080.10550144627810461No Hit
GCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT12040.10515210374075988No Hit
TGTTATTCGATTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC12030.10506476810642372No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC11990.104715425569079No Hit
TGATAATCTCAAGACACCTCCCTTAGCTACTCAGGAGGCTGAGGCAGAAA11990.104715425569079No Hit
AGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGC11900.10392940486005338No Hit
GACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA11860.10358006232270867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11820.10323071978536394No Hit
GAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGAT11670.10192068527032126No Hit
CGGATGATAATCTCAAGACACGTGCCGAGTGTCTGCTCCATCCCCTTCCA11590.10122200019563182No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG11560.1009599932926233No Hit
GGCTCAGCAGGGAGCTGGTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGAC11550.1008726576582871No Hit
ACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGC11540.10078532202395094No Hit
TGGATGTGTCTGTGCCCAAGGTAGAAGGTGAAATGAAAGTGCCAGATGTT11490.10034864385227002No Hit
AATGAAAGTGATGATGAGGAAGAGGAAGAAAAAGGGCCAGTGTCTCCCAG11470.10017397258359768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)