FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003249941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866484
Sequences flagged as poor quality0
Sequence length126
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT59720.31995988178843215No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA40820.21869997278305092No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC35520.19030433692439902No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC31980.17133819523767682No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC29840.15987278755135323No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA29440.1577297206940965No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT29280.1568724939511938No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT28760.15408650703676002No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC27460.1471215397506756No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG27210.14578212296489015No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC25650.13742416222158882No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC24860.13319160517850676No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA23540.12611948454955949No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC22580.1209761240921433No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA22480.12044035737782911No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA22300.11947597729206358No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT21640.11593991697758994No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT21360.11443977017751024No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT21330.11427904016321597No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC21300.11411831014892172No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG21170.11342181342031328No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC20310.10881421967721128No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC20290.10870706633434844No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC20110.1077426862485829No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC20000.1071533428628373No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT20000.1071533428628373No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC19880.10651042280566028No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC19810.10613538610564034No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT19080.10222428909114677No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT18710.10024195224818427No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC18690.10013479890532144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)