FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318897754
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG65468072.052948607471221TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC25458950.041.149689
GAGCACA25713050.040.741918
AGAGCAC25790100.040.6381267
CGGAAGA26699900.039.271483
TCGGAAG27067950.038.766142
GAAGAGC27293850.038.4534655
ATCGGAA27429100.038.2132381
AAGAGCA28583350.036.739616
GGAAGAG30001500.035.0462464
CGTCTTC12694450.016.53514150-54
ATGCCGT12863300.016.3663945-49
TATGCCG12937350.016.31272745-49
GCCGTCT12681450.016.25971450-54
TGCCGTC12803800.016.25908545-49
CTCGTAT12849100.015.95971340-44
CCGTCTT13114100.015.93812850-54
TCTCGTA13021900.015.74581140-44
TCGTATG13135600.015.48995940-44
CGTATGC13371500.015.4836645-49
ATCTCGT13403950.015.22723340-44
Produced by FastQC (version 0.11.5)