FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003249951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003249951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318897754
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC65880012.065866227455462Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG23052000.044.618119
GAGCGTC23761800.043.3128748
AGAGCGT24633000.041.9016847
AAGAGCG25839750.040.0478486
CGGAAGA26762450.038.720493
TCGGAAG27106700.038.236892
ATCGGAA27462450.037.6840171
GAAGAGC27608750.037.5382655
GGAAGAG30713450.033.8707124
CGCCGTA11677900.017.80611855-59
GCCGTAT11747550.017.69783455-59
CGTATCA11780150.017.60174260-64
TCGCCGT11816550.017.57547255-59
CCGTATC11776200.017.53510555-59
GTCGCCG12132150.017.0556555-59
TGGTCGC12480000.016.7012450-54
GGTCGCC12409900.016.66800550-54
GTGGTCG12692800.016.48360350-54
GTATCAT13346550.015.80262360-64
CTCGGTG13345450.015.67512545-49
Produced by FastQC (version 0.11.5)